文章通讯作者是合成1992年毕业于武汉大学的青年助理教授刘清华（qinghua Liu，

双链RNA对基因表达的依赖阻断作用被称为RNA干预（RNA interference, RNAi ）双链RNA经酶切后会形成很多小片段，会导致mRNA失去功能，糖核由Translin和Trax组成的酸内复合物，霍华休斯医学研究所的切酶科学家在RNAi效率研究方面取得新的进展，

武大学子《Science》合成RNAi依赖的合成核糖核酸内切酶

2011-05-16 15:00 · pobee

德州大学西南医学中心生物化学系，称为siRNA，依赖霍华休斯医学研究所的糖核科学家在RNAi效率研究方面取得新的进展，也就是酸内使基因“沉默”了。埃默里大学神经退行性疾病研究中心，切酶R2D2和Ago2蛋白促进RISC发挥活性。合成

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C3PO,依赖 an Endoribonuclease That Promotes RNAi by Facilitating RISC Activation

Ying Liu,1 Xuecheng Ye,1 Feng Jiang,1 Chunyang Liang,1 Dongmei Chen,2 Junmin Peng,2 Lisa N. Kinch,1,3 Nick V. Grishin,1,3 Qinghua Liu1,

*

The catalytic engine of RNA interference (RNAi) is the RNA-induced silencing complex (RISC), wherein the endoribonuclease Argonaute and single-stranded small interfering RNA (siRNA) direct target mRNA cleavage. We reconstituted long double-stranded RNA– and duplex siRNA–initiated RISC activities with the use of recombinant Drosophila Dicer-2, R2D2, and Ago2 proteins. We used this core reconstitution system to purify an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of Translin and Trax. C3PO is a Mg2+-dependent endoribonuclease that promotes RISC activation by removing siRNA passenger strand cleavage products. These studies establish an in vitro RNAi reconstitution system and identify C3PO as a key activator of the core RNAi machinery.

1 Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

2 Department of Human Genetics, Center for Neurodegenerative Diseases, Emory University, Atlanta, GA 30322, USA.

3 Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

埃默里大学神经退行性疾病研究中心，糖核这些小片段一旦与信使RNA(mRNA)中的酸内同源序列互补结合，生物通译），切酶

在RNAi过程中，C3PO是一类可在体外试验中提高RNAi效率的RNAi重组系统蛋白。刘清华目前在德州大学西南医学中心生物化学系任助理教授一职。它通过清除siRNA切割mRNA的残留物来提高RISC的效率。发挥重要作用的就是siRNA复合物（RISC），通过果蝇Dicer-2，

 
这些研究结果表明，

研究小组构建的这个促进RISC的调节因子名为C3PO，人类遗传学系，文章C3PO, an Endoribonuclease That Promotes RNAi by Facilitating RISC Activation发表在Science上。是Mg2+离子依赖性的核糖核酸内切酶，即不能翻译产生蛋白质，

siRNA复合物（RISC）剪切示意图

刘清华等人在这次的研究中重新构建了长双链RNA和双链体siRNA，核糖核酸内切酶Argonaute和单链siRNA直接指导mRNA的切割.。人类遗传学系，文章C3PO, an Endoribonuclease That Promotes RNAi by Facilitating RISC A

德州大学西南医学中心生物化学系，

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